The following sites cut once in the GR ligand binding domain

Sequence : hGRalpha lbf seq ( 1 > 833 ), 8 Unique SitesSequence
Enzymes : 8 of 146 enzymes (Filtered), 8 Enzymes Cut
Settings : Linear, Certain Sites Only, Standard Genetic Code
734 Ava I
663 Ava III
351 BspM I
728 EcoR I
56 Mae II
663 Nsi I
276 Pst I
776 Ssp I