General Lab Protocols

Recipes

Microdissection

Microinjection

DNA Transgenics

• BAC
• Meganuclease
• Sperm-mediated

Ventricles

Microscopy

Confocal

Molecular Biology Protocols

Bacterial Transformations

Northern Blots

Northern Blots fro MicroRNA's

Plasmid Organization

• Stocks

Plasmid Preps

PCR

• General PCR set up for zebrafish
• Single cell PCR for zebrafish embryos

RT-PCR

• RT-PCR for zebrafish embryos
• RT-PCR from zebrafish explants
• RT-PCR for frogs

Restriction Digests

Southern Blots

Western Blots

Mutagenesis

Mutagenesis Protocol from Stratagene

Staining

H & E Staining

Histology for Frogs

Immunocytochemistry

• Antibodies
• Acetylated tubulin and RM044 immunostaining

In situ Hybridization

• In situ protocol for zebrafish embryos
• Frog in situ hybridization protocol

TUNEL